Measuring Ligand Binding Kinetics to Membrane Proteins Using Virion Nano-oscillators.

ABSTRACT: Membrane proteins play vital roles in cellular signaling processes and serve as the most popular drug targets. A key task in studying cellular functions and developing drugs is to measure the binding kinetics of ligands with the membrane proteins. However, this has been a long-standing challenge because one must perform the measurement in a membrane environment to maintain the conformations and functions of the membrane proteins. Here, we report a new method to measure ligand binding kinetics to membrane proteins using self-assembled virion oscillators. Virions of human herpesvirus were used to display human G-protein-coupled receptors (GPCRs) on their viral envelopes. Each virion was then attached to a gold-coated glass surface via a flexible polymer to form an oscillator and driven into

SUBMITTER: Ma G 

PROVIDER: S-EPMC6211285 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature