H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4α⁺ Periportal Hepatocytes but Not Cytokeratin 19⁺ Cholangiocytes in Cholestatic Livers.

ABSTRACT: Long noncoding RNA (lncRNA) H19 is abundantly expressed in fetal liver. Its expression is significantly diminished in adult healthy liver but is re-induced in chronic liver diseases, including cholestasis. In this study, we developed a new method with combined in situ hybridization (ISH) and immunofluorescence (IF) colabeling to establish an H19 expression profile with both parenchymal and nonparenchymal cell-specific markers in the livers of cholestatic mouse models and patients with cholestasis. H19RNA⁺ cells showed no colocalization with biliary epithelial cell marker cytokeratin 19 (CK19)⁺ cholangiocytes but were immediately adjacent to biliary structures in bile duct ligation (BDL), 3,5-diethoxycarbony1-1,4-dihydrocollidine (DDC), and multidr

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC6211330 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature