Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae).

ABSTRACT:

Background

Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement.

Results

Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively.

Conclusions

From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for an

SUBMITTER: Garcia-Gonzales R 

PROVIDER: S-EPMC6211410 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature