Phytophthora methylomes are modulated by 6mA methyltransferases and associated with adaptive genome regions.

ABSTRACT:

Background

Filamentous plant pathogen genomes often display a bipartite architecture with gene-sparse, repeat-rich compartments serving as a cradle for adaptive evolution. The extent to which this two-speed genome architecture is associated with genome-wide DNA modifications is unknown.

Results

We show that the oomycetes Phytophthora infestans and Phytophthora sojae possess functional adenine N6-methylation (6mA) methyltransferases that modulate patterns of 6mA marks across the genome. In contrast, 5-methylcytosine could not be detected in these species. Methylated DNA IP sequencing (MeDIP-seq) of each species reveals 6mA is depleted around the transcription start sites (TSSs) and is associated with lowly expressed genes, particularly transposable elements. Genes occupying t

SUBMITTER: Chen H 

PROVIDER: S-EPMC6211444 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature