The proteomic and metabolomic characterization of exercise-induced sweat for human performance monitoring: A pilot investigation.

ABSTRACT: Sweat is a biofluid with several attractive attributes. However, investigation into sweat for biomarker discovery applications is still in its infancy. To add support for the use of sweat as a non-invasive media for human performance monitoring, volunteer participants were subjected to a physical exertion model using a treadmill. Following exercise, sweat was collected, aliquotted, and analyzed for metabolite and protein content via high-resolution mass spectrometry. Overall, the proteomic analysis illustrates significant enrichment steps will be required for proteomic biomarker discovery from single sweat samples as protein abundance is low in this medium. Furthermore, the results indicate a potential for protein degradation, or a large number of low molecular weight protein/peptides, in

SUBMITTER: Harshman SW 

PROVIDER: S-EPMC6211630 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature