Identification of grade and origin specific cell populations in serous epithelial ovarian cancer by single cell RNA-seq.

ABSTRACT: Here we investigated different cell populations within ovarian cancer using single-cell RNA seq: fourteen samples from nine patients with differing grades (high grade, low grade and benign) as well as different origin sites (primary and metastatic tumor site, ovarian in origin and fallopian in origin). We were able to identify sixteen distinct cell populations with specific cells correlated to high grade tumors, low grade tumors, benign and one population unique to a patient with a breast cancer relapse. Furthermore the proportion of these populations changes from primary to metastatic in a shift from mainly epithelial cells to leukocytes with few cancer epithelial cells in the metastases. Differential gene expression shows myeloid lineage cells are the primary cell group expressing solubl

SUBMITTER: Shih AJ 

PROVIDER: S-EPMC6211742 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature