Post-Translational Modifications in Polypyrimidine Tract Binding Proteins PTBP1 and PTBP2.

ABSTRACT: RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns and regulate different sets of target exons. How highly similar members in a gene family can exert different splicing outcomes is not well understood. We conducted mass spectrometry analysis of post-translational phosphorylation and acetylation modifications for two paralogs of the polypyrimidine tract binding protein family, PTBP1 and PTBP2, to discover modifications that occur in splicing reaction mixtures and to identify discrete modifications that may direct their different

SUBMITTER: Pina JM 

PROVIDER: S-EPMC6211845 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature