Genome-Scale Analysis of Perturbations in Translation Elongation Based on a Computational Model.

ABSTRACT: Perturbations play an important role both in engineered systems and cellular processes. Thus, understanding their effect on protein synthesis should contribute to all biomedical disciplines. Here we describe the first genome-scale analysis of perturbations in translation-related factors in S. cerevisiae. To this end, we used simulations based on a computational model that takes into consideration the fundamental stochastic and bio-physical nature of translation. We found that the initiation rate has a key role in determining the sensitivity to perturbations. For low initiation rates, the first codons of the coding region dominate the sensitivity, which is highly correlated with the ratio between initiation rate and mean elongation rate (r = -0.95), with the open reading frame (ORF) length

SUBMITTER: Levin D 

PROVIDER: S-EPMC6212587 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature