Characterization analysis and heavy metal-binding properties of CsMTL3 in Escherichia coli.

ABSTRACT: Members of the metallothionein (MT) superfamily are involved in coordinating transition metal ions. In plants, MT family members are characterized by their arrangement of Cys residues. In this study, one member of the MT superfamily, CsMTL3, was characterized from a complementary DNA (cDNA) library from young cucumber fruit; CsMTL3 is predicted to encode a 64 amino acid protein with a predicted molecular mass of 6.751 kDa. Phylogenetic analysis identified it as a type 3 family member as the arrangement of N-terminal Cys residues was different from that of MT-like 2. Heterologous expression of CsMTL3 in Escherichia coli improved their heavy metal tolerance, particularly to Cd²⁺ and Cu²⁺, and led to increased uptake of Cd²⁺ and Cu^2+<

SUBMITTER: Xu X 

PROVIDER: S-EPMC6212650 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature