Deep genome annotation of the opportunistic human pathogen Streptococcus pneumoniae D39.

ABSTRACT: A precise understanding of the genomic organization into transcriptional units and their regulation is essential for our comprehension of opportunistic human pathogens and how they cause disease. Using single-molecule real-time (PacBio) sequencing we unambiguously determined the genome sequence of Streptococcus pneumoniae strain D39 and revealed several inversions previously undetected by short-read sequencing. Significantly, a chromosomal inversion results in antigenic variation of PhtD, an important surface-exposed virulence factor. We generated a new genome annotation using automated tools, followed by manual curation, reflecting the current knowledge in the field. By combining sequence-driven terminator prediction, deep paired-end transcriptome sequencing and enrichment of primary tran

SUBMITTER: Slager J 

PROVIDER: S-EPMC6212727 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature