A Secreted Bacterial Peptidylarginine Deiminase Can Neutralize Human Innate Immune Defenses.

ABSTRACT: The keystone oral pathogen Porphyromonas gingivalis is associated with severe periodontitis. Intriguingly, this bacterium is known to secrete large amounts of an enzyme that converts peptidylarginine into citrulline residues. The present study was aimed at identifying possible functions of this citrullinating enzyme, named Porphyromonas peptidylarginine deiminase (PPAD), in the periodontal environment. The results show that PPAD is detectable in the gingiva of patients with periodontitis, and that it literally neutralizes human innate immune defenses at three distinct levels, namely bacterial phagocytosis, capture in neutrophil extracellular traps (NETs), and killing by the lysozyme-derived cationic antimicrobial peptide LP9. As shown by mass spectrometry, exposure of neutrop

SUBMITTER: Stobernack T 

PROVIDER: S-EPMC6212822 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature