Unknown

Dataset Information

0

Transcriptome Sequencing Reveals the Differentially Expressed lncRNAs and mRNAs Involved in Cryoinjuries in Frozen-Thawed Giant Panda (Ailuropoda melanoleuca) Sperm.


ABSTRACT: Sperm cryopreservation and artificial insemination are important methods for giant panda breeding and preservation of extant genetic diversity. Lower conception rates limit the use of artificial insemination with frozen-thawed giant panda sperm, due to the lack of understanding of the cryodamaging or cryoinjuring mechanisms in cryopreservation. Long non-coding RNAs (lncRNAs) are involved in regulating spermatogenesis. However, their roles during cryopreservation remain largely unexplored. Therefore, this study aimed to identify differentially expressed lncRNAs and mRNAs associated with cryodamage or freeze tolerance in frozen-thawed sperm through high throughput sequencing. A total of 61.05 Gb clean reads and 22,774 lncRNA transcripts were obtained. From the sequencing results, 1477 significantly up-regulated and 1,396 significantly down-regulated lncRNA transcripts from fresh and frozen-thawed sperm of giant panda were identified. GO and KEGG showed that the significantly dysregulated lncRNAs and mRNAs were mainly involved in regulating responses to cold stress and apoptosis, such as the integral component of membrane, calcium transport, and various signaling pathways including PI3K-Akt, p53 and cAMP. Our work is the first systematic profiling of lncRNA and mRNA in fresh and frozen-thawed giant panda sperm, and provides valuableinsights into the potential mechanism of cryodamage in sperm.

SUBMITTER: Ran MX 

PROVIDER: S-EPMC6212861 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Transcriptome Sequencing Reveals the Differentially Expressed lncRNAs and mRNAs Involved in Cryoinjuries in Frozen-Thawed Giant Panda (<i>Ailuropoda melanoleuca</i>) Sperm.

Ran Ming-Xia MX   Li Yuan Y   Zhang Yan Y   Liang Kai K   Ren Ying-Nan YN   Zhang Ming M   Zhou Guang-Bin GB   Zhou Ying-Min YM   Wu Kai K   Wang Cheng-Dong CD   Huang Yan Y   Luo Bo B   Qazi Izhar Hyder IH   Zhang He-Min HM   Zeng Chang-Jun CJ  

International journal of molecular sciences 20181008 10


Sperm cryopreservation and artificial insemination are important methods for giant panda breeding and preservation of extant genetic diversity. Lower conception rates limit the use of artificial insemination with frozen-thawed giant panda sperm, due to the lack of understanding of the cryodamaging or cryoinjuring mechanisms in cryopreservation. Long non-coding RNAs (lncRNAs) are involved in regulating spermatogenesis. However, their roles during cryopreservation remain largely unexplored. Theref  ...[more]

Similar Datasets

| S-EPMC7916315 | biostudies-literature
| S-EPMC3885951 | biostudies-literature
| S-EPMC7142162 | biostudies-literature
| S-EPMC7111782 | biostudies-literature
| S-EPMC5658932 | biostudies-literature
| S-EPMC4580591 | biostudies-literature
| S-EPMC7112545 | biostudies-literature
| S-EPMC1904234 | biostudies-literature
| S-EPMC6879133 | biostudies-literature
| S-EPMC5050549 | biostudies-literature