Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus.

ABSTRACT: Human respiratory syncytial virus (RSV) is one of the most common viruses infecting the respiratory tracts of infants. The rapid and sensitive detection of RSV is important to minimize the incidence of infection. In this study, novel monoclonal antibodies (mAbs; B11A5 and E8A11) against RSV nucleoprotein (NP) were developed and applied to develop a rapid fluorescent immunochromatographic strip test (FICT), employing europium nanoparticles as the fluorescent material. For the FICT, the limits of detection of the antigen and virus were 1.25 µg/mL and 4.23 × 10⁶ TCID₅₀/mL, respectively, corresponding to 4.75 × 10⁶ ± 5.8 ×10⁵ (mean ± SD) RNA copy numbers per reaction mixture for RSV NP. A clinical study revealed a sensitivity of 90% (18/20) and specificity of 98.18% (108/110) for RS

SUBMITTER: Thuy Tien TT 

PROVIDER: S-EPMC6212954 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature