Differentially Expressed Genes Associated with the Cabbage Yellow-Green-Leaf Mutant in the ygl-1 Mapping Interval with Recombination Suppression.

ABSTRACT: Although the genetics and preliminary mapping of the cabbage yellow-green-leaf mutant YL-1 has been extensively studied, transcriptome profiling associated with the yellow-green-leaf mutant of YL-1 has not been discovered. Positional mapping with two populations showed that the yellow-green-leaf gene ygl-1 is located in a recombination-suppressed genomic region. Then, a bulk segregant RNA-seq (BSR) was applied to identify differentially expressed genes (DEGs) using an F₃ population (YL-1 × 11-192) and a BC₂ population (YL-1 × 01-20). Among the 37,286 unique genes, 5730 and 4118 DEGs were detected between the yellow-leaf and normal-leaf pools from the F₃ and BC₂ populations. BSR analysis with four pools greatly reduced the number of common DEGs from 4924 to 1112. In the ygl-1

SUBMITTER: Liu X 

PROVIDER: S-EPMC6212964 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature