Tuning Nanopore Diameter of Titanium Surfaces to Improve Human Gingival Fibroblast Response.

ABSTRACT: The aim of this study was to determine the optimal nanopore diameter of titanium nanostructured surfaces to improve human gingival fibroblast (hGF) response, with the purpose of promoting gingiva integration to dental implant abutments. Two TiO₂ nanoporous groups with different diameters (NP-S ~48 nm and NP-B ~74 nm) were grown on Ti foils using an organic electrolyte containing fluoride by electrochemical oxidation, varying the applied voltage and the interelectrode spacing. The surfaces were characterized by scanning electron microscope (SEM), atomic force microscopy (AFM), and contact angle. The hGF were cultured onto the different surfaces, and metabolic activity, cytotoxicity, cell adhesion, and gene expression were analyzed. Bigger porous diameters (NP-B) were obtained by increasing

SUBMITTER: Ferra-Canellas MDM 

PROVIDER: S-EPMC6213077 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature