Photothermal Off-Resonance Tapping for Rapid and Gentle Atomic Force Imaging of Live Cells.

ABSTRACT: Imaging living cells by atomic force microscopy (AFM) promises not only high-resolution topographical data, but additionally, mechanical contrast, both of which are not obtainable with other microscopy techniques. Such imaging is however challenging, as cells need to be measured with low interaction forces to prevent either deformation or detachment from the surface. Off-resonance modes which periodically probe the surface have been shown to be advantageous, as they provide excellent force control combined with large amplitudes, which help reduce lateral force interactions. However, the low actuation frequency in traditional off-resonance techniques limits the imaging speed significantly. Using photothermal actuation, we probe the surface by directly actuating the cantilever. Due to the mu

SUBMITTER: Nievergelt AP 

PROVIDER: S-EPMC6213139 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature