Esrrb extinction triggers dismantling of naive pluripotency and marks commitment to differentiation.

ABSTRACT: Self-renewal of embryonic stem cells (ESCs) cultured in LIF/fetal calf serum (FCS) is incomplete with some cells initiating differentiation. While this is reflected in heterogeneous expression of naive pluripotency transcription factors (TFs), the link between TF heterogeneity and differentiation is not fully understood. Here, we purify ESCs with distinct TF expression levels from LIF/FCS cultures to uncover early events during commitment from naïve pluripotency. ESCs carrying fluorescent Nanog and Esrrb reporters show Esrrb downregulation only in Nanog^low cells. Independent Esrrb reporter lines demonstrate that Esrrb^negative ESCs cannot effectively self-renew. Upon Esrrb loss, pre-implantation pluripotency gene expression collapses. ChIP-Seq iden

SUBMITTER: Festuccia N 

PROVIDER: S-EPMC6213284 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature