Roles of orf60a and orf61 in Development of Bacteriophages λ and Φ24B.

ABSTRACT: The exo-xis region of lambdoid bacteriophage genomes contains several established and potential genes that are evolutionarily conserved, but not essential for phage propagation under laboratory conditions. Nevertheless, deletion or overexpression of either the whole exo-xis region and important regulatory elements can significantly influence the regulation of phage development. This report defines specific roles for orf60a and orf61 in bacteriophage λ and Φ24B, a specific Shiga toxin-converting phage with clinical relevance. We observed that mutant phages bearing deletions of orf60a and orf61 impaired two central aspects of phage development: the lysis-versus-lysogenization decision and prophage induction. These effects were more pronounced for phage Φ24B than for λ. Surprisingly, adsorpti

SUBMITTER: Dydecka A 

PROVIDER: S-EPMC6213356 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature