Histone Deacetylases Enhance Ca²⁺-Activated K⁺ Channel K_Ca3.1 Expression in Murine Inflammatory CD4⁺ T Cells.

ABSTRACT: The up-regulated expression of the Ca²⁺-activated K⁺ channel K_Ca3.1 in inflammatory CD4⁺ T cells has been implicated in the pathogenesis of inflammatory bowel disease (IBD) through the enhanced production of inflammatory cytokines, such as interferon-γ (IFN-γ). However, the underlying mechanisms have not yet been elucidated. The objective of the present study is to clarify the involvement of histone deacetylases (HDACs) in the up-regulation of K_Ca3.1 in the CD4⁺ T cells of IBD model mice. The expression levels of K_Ca3.1 and its regulators, such as function-modifying molecules and transcription factors, were quantitated using a real-time polymerase chain reaction (PCR) assay, Western blotting, and depolarization responses, which were induced by th

SUBMITTER: Matsui M 

PROVIDER: S-EPMC6213394 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature