Removal of dentin non-collagenous structures results in the unraveling of microfibril bundles in collagen type I.

ABSTRACT:

Aims

The structural organization of collagen from mineralized tissues, such as dentin and bone, has been a topic of debate in the recent literature. Recent reports have presented novel interpretations of the complexity of collagen type I at different hierarchical levels and in different tissues. Here, we investigate the nanostructural organization of demineralized dentin collagen following the digestion of non-collagenous components with a trypsin enzyme.

Materials and methods

Dentin specimens were obtained from healthy third-molars, cut into small cubes, and polished down to 1 µm roughness. Samples were then demineralized with 10% citric acid for 2 min. Selected specimens were further treated with a solution containing 1 mg/ml trypsin for 48 hours at 37 °C (pH 7.9-9.0). Bot

SUBMITTER: Bertassoni LE 

PROVIDER: S-EPMC6214662 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature