Quantitative Lipid Imaging Reveals a New Signaling Function of Phosphatidylinositol-3,4-Bisphophate: Isoform- and Site-Specific Activation of Akt.

ABSTRACT: Activation of class I phosphatidylinositol 3-kinase (PI3K) leads to formation of phosphatidylinositol-3,4,5-trisphophate (PIP₃) and phosphatidylinositol-3,4-bisphophate (PI34P₂), which spatiotemporally coordinate and regulate a myriad of cellular processes. By simultaneous quantitative imaging of PIP₃ and PI34P₂ in live cells, we here show that they have a distinctively different spatiotemporal distribution and history in response to growth factor stimulation, which allows them to selectively induce the membrane recruitment and activation of Akt isoforms. PI34P₂ selectively activates Akt2 at both the plasma membrane and early endosomes, whereas PIP₃ selectively stimulates Akt1 and Akt3 exclusively at the plasma membrane. The

SUBMITTER: Liu SL 

PROVIDER: S-EPMC6214670 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature