CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming.

ABSTRACT: Comprehensive identification of factors that can specify neuronal fate could provide valuable insights into lineage specification and reprogramming, but systematic interrogation of transcription factors, and their interactions with each other, has proven technically challenging. We developed a CRISPR activation (CRISPRa) approach to systematically identify regulators of neuronal-fate specification. We activated expression of all endogenous transcription factors and other regulators via a pooled CRISPRa screen in embryonic stem cells, revealing genes including epigenetic regulators such as Ezh2 that can induce neuronal fate. Systematic CRISPR-based activation of factor pairs allowed us to generate a genetic interaction map for neuronal differentiation, with confirmation of top individual an

SUBMITTER: Liu Y 

PROVIDER: S-EPMC6214761 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature