Proteomics based analysis of the nicotine catabolism in Paenarthrobacter nicotinovorans pAO1.

ABSTRACT: Paenarthrobacter nicotinovorans is a nicotine-degrading microorganism that shows a promising biotechnological potential for the production of compounds with industrial and pharmaceutical importance. Its ability to use nicotine was linked to the presence of the catabolic megaplasmid pAO1. Although extensive work has been performed on the molecular biology of nicotine degradation in this bacterium, only half of the genes putatively involved have been experimentally linked to nicotine. In the current approach, we used nanoLC-MS/MS to identify a total of 801 proteins grouped in 511 non-redundant protein clusters when P. nicotinovorans was grown on citrate, nicotine and nicotine and citrate as the only carbon sources. The differences in protein abundance showed that deamination is preferred whe

SUBMITTER: Mihasan M 

PROVIDER: S-EPMC6214936 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature