Methanol induces cytosolic calcium variations, membrane depolarization and ethylene production in arabidopsis and tobacco.

ABSTRACT:

Background and aims

Methanol is a volatile organic compound released from plants through the action of pectin methylesterases (PMEs), which demethylesterify cell wall pectins. Plant PMEs play a role in developmental processes but also in responses to herbivory and infection by fungal or bacterial pathogens. However, molecular mechanisms that explain how methanol could affect plant defences remain poorly understood.

Methods

Using cultured cells and seedlings from Arabidopsis thaliana and tobacco BY2 expressing the apoaequorin gene, allowing quantification of cytosolic Ca2+, a reactive oxygen species (ROS) probe (CLA, Cypridina luciferin analogue) and electrophysiological techniques, we followed early plant cell responses to exogenously supplied methanol applied as a liquid or

SUBMITTER: Tran D 

PROVIDER: S-EPMC6215043 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature