Prepubertal skeletal muscle growth requires Pax7-expressing satellite cell-derived myonuclear contribution.

ABSTRACT: The functional role of Pax7-expressing satellite cells (SCs) in postnatal skeletal muscle development beyond weaning remains obscure. Therefore, the relevance of SCs during prepubertal growth, a period after weaning but prior to the onset of puberty, has not been examined. Here, we have characterized mouse skeletal muscle growth during prepuberty and found significant increases in myofiber cross-sectional area that correlated with SC-derived myonuclear number. Remarkably, genome-wide RNA-sequencing analysis established that post-weaning juvenile and early adolescent skeletal muscle have markedly different gene expression signatures. These distinctions are consistent with extensive skeletal muscle maturation during this essential, albeit brief, developmental phase. Indelible labeling of SCs

SUBMITTER: Bachman JF 

PROVIDER: S-EPMC6215399 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature