Apolipoprotein E deficiency accelerates atherosclerosis development in miniature pigs.

ABSTRACT: Miniature pigs have advantages over rodents in modeling atherosclerosis because their cardiovascular system and physiology are similar to that of humans. Apolipoprotein E (ApoE) deficiency has long been implicated in cardiovascular disease in humans. To establish an improved large animal model of familial hypercholesterolemia and atherosclerosis, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 system (CRISPR/Cas9) was used to disrupt the ApoE gene in Bama miniature pigs. Biallelic-modified ApoE pigs with in-frame mutations (ApoE^m/m

SUBMITTER: Fang B 

PROVIDER: S-EPMC6215431 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature