Improvement of mosquito identification by MALDI-TOF MS biotyping using protein signatures from two body parts.

ABSTRACT:

Background

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology (MALDI-TOF MS) is an innovative tool that has been shown to be effective for the identification of numerous arthropod groups including mosquitoes. A critical step in the implementation of MALDI-TOF MS identification is the creation of spectra databases (DB) for the species of interest. Mosquito legs were the body part most frequently used to create identification DB. However, legs are one of the most fragile mosquito compartments, which can put identification at risk. Here, we assessed whether mosquito thoraxes could also be used as a relevant body part for mosquito species identification using a MALDI-TOF MS biotyping strategy; we propose a double DB query strategy to reinforce ident

SUBMITTER: Vega-Rua A 

PROVIDER: S-EPMC6215610 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature