Norepinephrine triggers an immediate-early regulatory network response in primary human white adipocytes.

ABSTRACT:

Background

Norepinephrine (NE) signaling has a key role in white adipose tissue (WAT) functions, including lipolysis, free fatty acid liberation and, under certain conditions, conversion of white into brite (brown-in-white) adipocytes. However, acute effects of NE stimulation have not been described at the transcriptional network level.

Results

We used RNA-seq to uncover a broad transcriptional response. The inference of protein-protein and protein-DNA interaction networks allowed us to identify a set of immediate-early genes (IEGs) with high betweenness, validating our approach and suggesting a hierarchical control of transcriptional regulation. In addition, we identified a transcriptional regulatory network with IEGs as master regulators, including HSF1 and NFIL3 as novel

SUBMITTER: Higareda-Almaraz JC 

PROVIDER: S-EPMC6215669 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature