CD8⁺ T-Cell Density Imaging with ⁶⁴Cu-Labeled Cys-Diabody Informs Immunotherapy Protocols.

ABSTRACT: Purpose: Noninvasive and quantitative tracking of CD8⁺ T cells by PET has emerged as a potential technique to gauge response to immunotherapy. We apply an anti-CD8 cys-diabody, labeled with ⁶⁴Cu, to assess the sensitivity of PET imaging of normal and diseased tissue.Experimental Design: Radiolabeling of an anti-CD8 cys-diabody (169cDb) with ⁶⁴Cu was developed. The accumulation of ⁶⁴Cu-169cDb was evaluated with PET/CT imaging (0, 5, and 24 hours) and biodistribution (24 hours) in wild-type mouse strains (n = 8/group studied with imaging and IHC or flow cytometry) after intravenous administration. Tumor-infiltrating CD8⁺ T cells in tumor-bearing mice treated with CpG and αPD-1 were quantified and mapped (n = 6

SUBMITTER: Seo JW 

PROVIDER: S-EPMC6215696 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature