FGF9/FGFR2 increase cell proliferation by activating ERK1/2, Rb/E2F1, and cell cycle pathways in mouse Leydig tumor cells.

ABSTRACT: Fibroblast growth factor 9 (FGF9) promotes cancer progression; however, its role in cell proliferation related to tumorigenesis remains elusive. We investigated how FGF9 affected MA-10 mouse Leydig tumor cell proliferation and found that FGF9 significantly induced cell proliferation by activating ERK1/2 and retinoblastoma (Rb) phosphorylations within 15 minutes. Subsequently, the expressions of E2F1 and the cell cycle regulators: cyclin D1, cyclin E1 and cyclin-dependent kinase 4 (CDK4) in G₁ phase and cyclin A1, CDK2 and CDK1 in S-G₂ /M phases were increased at 12 hours after FGF9 treatment; and cyclin B1 in G₂ /M phases were induced at 24 hours after FGF9 stimulation, whereas the phosphorylations of p53, p21 and p27 were not affected by FGF9. Moreover, FG

SUBMITTER: Chang MM 

PROVIDER: S-EPMC6215879 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature