Inducible Intestine-Specific Expression of kras^V12 Triggers Intestinal Tumorigenesis In Transgenic Zebrafish.

ABSTRACT: KRAS mutations are a major risk factor in colorectal cancers. In particular, a point mutation of KRAS of amino acid 12, such as KRAS^V12, renders it stable activity in oncogenesis. We found that kras^V12 promotes intestinal carcinogenesis by generating a transgenic zebrafish line with inducible kras^V12 expression in the intestine, Tg(ifabp:EGFP-kras^V12). The transgenic fish generated exhibited significant increases in the rates of intestinal epithelial outgrowth, proliferation, and cross talk in the active Ras signaling pathway involving in epithelial-mesenchymal transition (EMT). These results provide in vivo evidence of Ras pathway activation via kras^V12 overexpression. Long-term transgenic expression of kras^V12 resulted in

SUBMITTER: Lu JW 

PROVIDER: S-EPMC6215966 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature