Detection of α-, β-, and γ-amanitin in urine by LC-MS/MS using ¹⁵N₁₀-α-amanitin as the internal standard.

ABSTRACT: The majority of fatalities from poisonous mushroom ingestion are caused by amatoxins. To prevent liver failure or death, it is critical to accurately and rapidly diagnose amatoxin exposure. We have developed a liquid chromatography tandem mass spectrometry method to detect α-, β-, and γ-amanitin in urine to meet this need. Two internal standard candidates were evaluated, including an isotopically labeled ¹⁵N₁₀-α-amanitin and a modified amanitin methionine sulfoxide synthetic peptide. Using the ¹⁵N₁₀-α-amanitin internal standard, precision and accuracy of α-amanitin in pooled urine was ≤5.49% and between 100 and 106%, respectively, with a reportable range from 1-200 ng/mL. β- and γ-Amanitin were most accurately quantitated in pooled urine using ex

SUBMITTER: Abbott NL 

PROVIDER: S-EPMC6216022 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature