Generation of Functional Hepatocytes from Human Adipose-Derived MYC⁺ KLF4⁺ GMNN⁺ Stem Cells Analyzed by Single-Cell RNA-Seq Profiling.

ABSTRACT: Cell transplantation holds considerable promise for end-stage liver diseases but identifying a suitable, transplantable cell type has been problematic. Here, we describe a novel type of mesenchymal stem cells (MSCs) from human adipose tissue. These cells are different from previously reported MSCs, they are in the euchromatin state with epigenetic multipotency, and express pluripotent markers MYC, KLF4, and GMNN. Most of the genes associated with germ layer specification are modified by H3K4me3 or co-modified by H3K4me3 and H3K27me3. We named this new type of MSCs as adult multipotent adipose-derived stem cells (M-ADSCs). Using a four-step nonviral system, M-ADSCs can be efficiently Induced into hepatocyte like cells with expression of hepatocyte markers, drug metabolizing enzymes and tran

SUBMITTER: Li H 

PROVIDER: S-EPMC6216430 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature