Uncovering Natural Longevity Alleles from Intercrossed Pools of Aging Fission Yeast Cells.

ABSTRACT: Quantitative traits often show large variation caused by multiple genetic factors . One such trait is the chronological lifespan of non-dividing yeast cells, serving as a model for cellular aging. Screens for genetic factors involved in aging typically assay mutants of protein-coding genes. To identify natural genetic variants contributing to cellular aging, we exploited two strains of the fission yeast, Schizosaccharomyces pombe, that differ in chronological lifespan. We generated segregant pools from these strains and subjected them to advanced intercrossing over multiple generations to break up linkage groups. We chronologically aged the intercrossed segregant pool, followed by genome sequencing at different times to detect genetic variants that became reproducibly enriched as a

SUBMITTER: Ellis DA 

PROVIDER: S-EPMC6216586 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature