Transient Expression of Biologically Active Anti-rabies Virus Monoclonal Antibody in Tobacco Leaves.

ABSTRACT:

Background

Rabies virus is a neurotropic virus that causes fatal, but, a preventable disease in mammals. Administration of rabies immunoglobulin (RIG) is essential for the post-exposure of the prophylaxis to prevent the disease. However, replacement of polyclonal RIGs with alternative monoclonal antibodies (MAbs) that are capable of neutralizing rabies virus has been recommended.

Objectives

Here, we have investigated the transient expression of the full-size human MAb against rabies virus glycoprotein; the MAb SO57 in the tobacco plants using vacuum agro-infiltration. Previously, stably transformed plants expressing the MAb have been reported.

Materials and methods

In this study three vectors carrying the codon-optimized genes for the heavy or light chain and p19 sile

SUBMITTER: Shafaghi M 

PROVIDER: S-EPMC6217261 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature