EXTH-45. THE REPURPOSED CUSP9 REGIMEN EXERTS POTENT ANTI-NEOPLASTIC ACTIVITY AGAINST GLIOBLASTOMA CELLS IN VITRO

ABSTRACT: Abstract

OBJECTIVE

Drug repurposing is a strategy to safely accelerate the clinical application of therapeutics that provide anti-cancer activity. CUSP9 represents such a therapeutic regime which includes nine repurposed drugs along with low-dose temozolomide. In this work, we examined the biological activity of the CUSP9 regimen in an in vitro setting of glioblastoma.

METHODS

MTT and soft-agar assays were used to examine cellular proliferation. Staining for Annexin V/PI and Western blotting were used to examine pro-apoptotic effects. A spheroid assay was performed to assess effects of the treatments on three-dimensional growth. Time-lapse microscopy, radius and transwell assays were performed to examine anti-migratory effects.

RESULTS

Treatment with CUSP9 exerte

SUBMITTER: Halatsch M 

PROVIDER: S-EPMC6217344 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature