Mechanism of tRNA-mediated +1 ribosomal frameshifting.

ABSTRACT: Accurate translation of the genetic code is critical to ensure expression of proteins with correct amino acid sequences. Certain tRNAs can cause a shift out of frame (i.e., frameshifting) due to imbalances in tRNA concentrations, lack of tRNA modifications or insertions or deletions in tRNAs (called frameshift suppressors). Here, we determined the structural basis for how frameshift-suppressor tRNA^SufA6 (a derivative of tRNA^Pro) reprograms the mRNA frame to translate a 4-nt codon when bound to the bacterial ribosome. After decoding at the aminoacyl (A) site, the crystal structure of the anticodon stem-loop of tRNA^SufA6 bound in the peptidyl (P) site reveals ASL conformational changes that allow for recoding into the +1 mRNA frame. Furthermore, a crystal str

SUBMITTER: Hong S 

PROVIDER: S-EPMC6217423 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature