EXTH-54. Bcl-2/Bcl-xL INHIBITION SYNERGISTICALLY ENHANCES THE ANTI-NEOPLASTIC ACTIVITY OF CUSP9 AGAINST GLIOBLASTOMA CELLS IN VITRO

ABSTRACT: Abstract

OBJECTIVE

Repurposing represents a promising approach to safely accelerate the clinical application of therapeutics with anti-cancer activity. In this study, we examined whether inhibition of the anti-apoptotic Bcl-2 family proteins Bcl-2 and Bcl-xL enhances the biological effects of the repurposed CUSP9 regimen in an in vitro setting of glioblastoma.

METHODS

We applied MTT assays to assess cellular proliferation. Annexin V/PI and TMRE staining were used to examine apoptosis. Western blotting, RT-PCR and specific knockdown experiments using siRNA were employed to examine molecular mechanisms of action.

RESULTS

Bcl-2/Bcl-xL inhibition by the BH3 mimetic ABT263, yielded synergistic anti-proliferative effects across a wide panel of established and primary c

SUBMITTER: Halatsch M 

PROVIDER: S-EPMC6217449 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature