Hydrogel Environment Supports Cell Culture Expansion of a Grade IV Astrocytoma.

ABSTRACT: Malignant astrocytomas are aggressive cancers of glial origin that can develop into invasive brain tumors. The disease has poor prognosis and high recurrence rate. Astrocytoma cell lines of human origin are an important tool in the experimental pathway from bench to bedside because they afford a convenient intermediate system for in vitro analysis of brain cancer pathogenesis and treatment options. We undertook the current study to determine whether hydrogel culture methods could be adapted to support the growth of astrocytoma cell lines, thereby facilitating a system that may be biologically more similar to in vivo tumor tissue. Our experimental protocols enabled maintenance of Grade IV astrocytoma cell lines in conventional monolayer culture and in the extracellular matrix hydrogel, Gelt

SUBMITTER: Jogalekar MP 

PROVIDER: S-EPMC6217807 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature