Characterization of a B16-F10 melanoma model locally implanted into the ear pinnae of C57BL/6 mice.

ABSTRACT: The common experimental use of B16-F10 melanoma cells focuses on exploring their metastatic potential following intravenous injection into mice. In this study, B16-F10 cells are used to develop a primary tumor model by implanting them directly into the ears of C57BL/6J mice. The model represents a reproducible and easily traceable tool for local tumor growth and for making additional in vivo observations, due to the localization of the tumors. This model is relatively simple and involves (i) surgical opening of the ear skin, (ii) removal of a square-piece of cartilage followed by (iii) the implantation of tumor cells with fibrin gel. The remodeling of the fibrin gel within the cartilage chamber, accompanying tumor proliferation, results in the formation of blood vessels, lymphatics and tis

SUBMITTER: Potez M 

PROVIDER: S-EPMC6218054 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature