A Robust Methodology for Assessing Differential Homeolog Contributions to the Transcriptomes of Allopolyploids.

ABSTRACT: Polyploidy has played a pivotal and recurring role in angiosperm evolution. Allotetraploids arise from hybridization between species and possess duplicated gene copies (homeologs) that serve redundant roles immediately after polyploidization. Although polyploidization is a major contributor to plant evolution, it remains poorly understood. We describe an analytical approach for assessing homeolog-specific expression that begins with de novo assembly of parental transcriptomes and effectively (i) reduces redundancy in de novo assemblies, (ii) identifies putative orthologs, (iii) isolates common regions between orthologs, and (iv) assesses homeolog-specific expression using a robust Bayesian Poisson-Gamma model to account for sequence bias when mapping polyploid reads back to p

SUBMITTER: Boatwright JL 

PROVIDER: S-EPMC6218233 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature