Correlated motions of C'-N and C_α-C_β pairs in protonated and per-deuterated GB3.

ABSTRACT: We investigated correlated µs-ms time scale motions of neighboring ¹³C'-¹⁵N and ¹³C_α-¹³C_β nuclei in both protonated and perdeuterated samples of GB3. The techniques employed, NMR relaxation due to cross-correlated chemical shift modulations, specifically target concerted changes in the isotropic chemical shifts of the two nuclei associated with spatial fluctuations. Field-dependence of the relaxation rates permits identification of the parameters defining the chemical exchange rate constant under the assumption of a two-site exchange. The time scale of motions falls into the intermediate to fast regime (with respect to the chemical shift time scale, 100-400 s^-1 range) for the ¹³C'-¹⁵N pairs a

SUBMITTER: Vugmeyster L 

PROVIDER: S-EPMC6218248 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature