Precise Delineation and Transcriptional Characterization of Bovine Blood Dendritic-Cell and Monocyte Subsets.

ABSTRACT: A clear-cut delineation of bovine bona fide dendritic cells (DC) from monocytes has proved challenging, given the high phenotypic and functional plasticity of these innate immune cells and the marked phenotypic differences between species. Here, we demonstrate that, based on expression of Flt3, CD172a, CD13, and CD4, a precise identification of bovine blood conventional DC type 1 and 2 (cDC1, cDC2), plasmacytoid DC (pDC), and monocytes is possible with cDC1 being Flt3⁺CD172a^dimCD13⁺CD4^-, cDC2 being Flt3⁺CD172a⁺CD13^-CD4^-, pDC being Flt3⁺CD172a^dimCD13^-CD4⁺, and monocytes being Flt3^-CD172a^highCD13^-CD4^-. The pheno

SUBMITTER: Talker SC 

PROVIDER: S-EPMC6218925 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature