Zoonotic Cryptosporidium species and subtypes in lambs and goat kids in Algeria.

ABSTRACT:

Background

Little is known on the occurrence and identity of Cryptosporidium species in sheep and goats in Algeria. This study aimed at investigating the occurrence of Cryptosporidium species in lambs and goat kids younger than 4 weeks.

Methods

A total of 154 fecal samples (62 from lambs and 92 from kid goats) were collected from 13 sheep flocks in Médea, Algeria and 18 goat flocks across Algiers and Boumerdes. They were screened for Cryptosporidium spp. by nested-PCR analysis of a fragment of the small subunit (SSU) rRNA gene, followed by restriction fragment length polymorphism and sequence analyses to determine the Cryptosporidium species present. Cryptosporidium parvum and C. ubiquitum were further subtyped by sequence analysis of the 60 kDa glycoprotein gene.

Results

SUBMITTER: Baroudi D 

PROVIDER: S-EPMC6219180 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature