Comprehensive transcriptome analysis of Sarcophaga peregrina, a forensically important fly species.

ABSTRACT: Sarcophaga peregrina (flesh fly) is a frequently found fly species in Palaearctic, Oriental, and Australasian regions that can be used to estimate minimal postmortem intervals important for forensic investigations. Despite its forensic importance, the genome information of S. peregrina has not been fully described. Therefore, we generated a comprehensive gene expression dataset using RNA sequencing and carried out de novo assembly to characterize the S. peregrina transcriptome. We obtained precise sequence information for RNA transcripts using two different methods. Based on primary sequence information, we identified sets of assembled unigenes and predicted coding sequences. Functional annotation of the aligned unigenes was performed using the UniProt, Gene Ontology, and Kyoto Encyclopedi

SUBMITTER: Kim JY 

PROVIDER: S-EPMC6219405 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature