1α,25-dihydroxyvitamin D₃-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression.

ABSTRACT:

Aim

The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) and augmenting human CAMP induction.

Materials & methods

Nanofibrous wound dressings containing 1,25(OH)₂D₃ were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo.

Results

1,25(OH)₂D₃ was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)₂D₃ showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)₂D₃-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expr

SUBMITTER: Jiang J 

PROVIDER: S-EPMC6219435 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature