An intestinal paracellular pathway biased toward positively-charged macromolecules.

ABSTRACT: Lacking an effective mechanism to safely and consistently enhance macromolecular uptake across the intestinal epithelium, prospects for successful development of oral therapeutic peptide drugs remain unlikely. We previously addressed this challenge by identifying an endogenous mechanism that controls intestinal paracellular permeability that can be activated by a peptide, termed PIP 640, which can increase cellular levels of phosphorylated myosin light chain at position S¹⁹ (MLC-pS¹⁹). Apical application in vitro or luminal application in vivo was shown to increase macromolecular solute transport within minutes that recovered completely within a few hours after removal. We now examine the nature of PIP 640-mediated permeability changes. Confluent Caco-2 cell monolayer

SUBMITTER: Almansour K 

PROVIDER: S-EPMC6219475 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature