Emergence of a cholecystokinin/sulfakinin signalling system in Lophotrochozoa.

ABSTRACT: Chordate gastrin/cholecystokinin (G/CCK) and ecdysozoan sulfakinin (SK) signalling systems represent divergent evolutionary scenarios of a common ancestral signalling system. The present article investigates for the first time the evolution of the CCK/SK signalling system in a member of the Lophotrochozoa, the second clade of protostome animals. We identified two G protein-coupled receptors (GPCR) in the oyster Crassostrea gigas (Mollusca), phylogenetically related to chordate CCK receptors (CCKR) and to ecdysozoan sulfakinin receptors (SKR). These receptors, Cragi-CCKR1 and Cragi-CCKR2, were characterised functionally using a cell-based assay. We identified di- and mono-sulphated forms of oyster Cragi-CCK1 (pEGAWDY(SO₃H)DY(SO₃H)GLGGGRF-NH₂) as the potent e

SUBMITTER: Schwartz J 

PROVIDER: S-EPMC6219549 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature