Numerical Simulation of Biomass Growth in OKTOP®9000 Reactor at Industrial Scale.

ABSTRACT: Computational fluid dynamics is a powerful method for scale-up of reactors although it is still challenging to fully embrace hydrodynamics and biological complexities. In this article, an aerobic fermentation of Pichia pastoris cells is modeled in a batch OKTOP®9000 reactor. The 800 m³ industrial scale reactor is equipped with a radial impeller, designed by Outotec Oy for gas dispersion in the draft tube reactor. Measured N _p of the impeller is used in hydrodynamics validation. The resolved energy dissipation rate is compensated, and its influence on mass transfer is analyzed and discussed. Gas-liquid drag force is modified to simulate effects of liquid turbulence and bubble swarms. Resolved steady state multiphase hydrodynamics is used to simulat

SUBMITTER: Gradov DV 

PROVIDER: S-EPMC6219852 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature