Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation.

ABSTRACT: Recently, genome editing in mouse zygotes has become convenient and scalable, in association with various technological developments and improvements such as novel nuclease tools, alternative delivery methods, and contemporary reproductive engineering techniques. We have so far demonstrated the applicability of ultra-superovulation, in vitro fertilization (IVF), and vitrification/warming of zygotes in microinjection-mediated mouse genome editing. Moreover, an electroporation-mediated method has rapidly become established for simple gene knockout and small precise modifications including single amino acid substitutions. Here, we present an updated example of an application coupling the following three latest technologies: 1) CRISPR-Cas9 ribonucleoprotein as the most convenient genome-editin

SUBMITTER: Nakagawa Y 

PROVIDER: S-EPMC6219886 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature